Table 1.
Oligonucleotide primers used for qPCR in this study
| Target gene | Forward/reverse | Sequence (5′-3′) | Primer name | Target size (bp) | PCR efficiency |
|---|---|---|---|---|---|
| rhlA | F | GCGAGTACATTCTGACGAAGG | rhlA1q F | 87 | 1.967 |
| R | ACACCGACAGCAGGAAACTC | rhlA1q R | |||
| rhlB | F | ACGAGGCGATGGCTAAAG | rhlBq F | 119 | 1.936 |
| R | ATGCGTGCAGAACACCAC | rhlBq R | |||
| rhlC | F | ATGCATCACGGGTGGTTG | rhlC1q F | 114 | 1.907 |
| R | CCATATCGTCAGCAGATTCG | rhlC1q R | |||
| gyrB | F | ATCCGACGATCTTCCACATC | gyrBq F | 92 | 1.954 |
| R | CAGCACGTTTTCGTTGTAGC | gyrBq R | |||
| rpoD | F | ACCGTCGTGGCTACAAATTC | rpoDq F | 117 | 1.919 |
| R | TCGTCTCGATCATGTGAACC | rpoDq R | |||
| rpoB | F | TTCGTGAGCTATGCGTTGTC | rpoBq F | 175 | 2.292 |
| R | TTTCGCCCATGTACACTTCC | rpoBq R |