Table 1.

Expression of recombinant human insulin in Pichia pastoris as evaluated by ELISA.

Commercial insulin		Time (hrs)	Recombinant proinsulin
Protein conc. (µg/mL)	OD 495		Mut+ clone		MutS clone
			OD 495	Protein conc. (µg/mL)	OD 495	Protein conc. (µg/mL)
5	0.695 ± 0.031	0	0.447 ± 0.015	—	0.473 ± 0.020	—
10	0.706 ± 0.015	24	0.523 ± 0.010	8	0.587 ± 0.120	11
15	0.776 ± 0.015	72	0.749 ± 0.023	20	0.0818 ± 0.021	26
20	0.798 ± 0.070	96	1.155 ± 0.130	31	1.205 ± 0.101	35
25	0.802 ± 0.110	144	0.870 ± 0.030	23	1.015 ± 0.060	29
30	0.870 ± 0.09

Mut⁺: methanol utilization +ve clone.

Mut^S: methanol utilization slow phenotype.