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MBD-score is bimodally distributed and correlates with CpGo/e. (A) Distribution of MBD-score (log2-fold difference between enriched and flow-through MBD-seq libraries). Higher values indicate stronger methylation. (B) Scatter plot of MBD-score and CpGo/e. Lower values for CpGo/e are expected with stronger methylation. Asterisks indicate significance based on Spearman’s rank-order correlation test (ns >0.05; * <0.05; ** <0.01; *** <0.001; **** <0.0001).
