Mitochondrial protein import proteins in various taxa. Dark blue: free-living mitochondrion; pale blue: free-living MRO; dark pink: parasite, mitochondrion; light pink, parasite, MRO; purple: facultative parasite, mitochondrion; dark grey: bacterium; pale grey: incomplete data. Data from Neupert (1997), Hoogenraad et al. (2002), Henriquez et al. (2005), Regoes et al. (2005), van der Laan et al. (2005); Williams and Keeling (2005), Dolezal et al. (2006), Figueroa-Martinez et al. (2008), Atteia et al. (2009), Dagley et al. (2009), Waller et al. (2009), Barberà et al. (2010), Dolezal et al. (2010), Jedelsky et al. (2011), Liu et al. (2011), Schneider et al. (2011), Eckers et al. (2012), Pusnik et al. (2012), Basu et al. (2013), Heinz and Lithgow (2013), Jerlstrom-Hultqvist et al. (2013), Wideman et al. (2013), Zubacova et al. (2013), Gawryluk et al. (2014), Murcha, Kmiec, et al. 2014; Murcha, Wang, et al. 2014; Stairs et al. (2014), Martincova et al. (2015), Murcha et al. (2015), Noguchi et al. (2015), Wojtkowska et al. (2015), and Buczek et al. (2016) this study. 1Transcriptome data, incomplete, particularly in the cases of S. marylandensis and Paratrimastix pyriformis; 2pATOM36; 3Tom9; 4pATOM; 5Metaxin 6Tim8/13; 7Tim17/22/23. For accession numbers and additional proteins, see supplementary table S4, Supplementary Material online.