Fig. 4.

Regulation of meiosis, ROS scavenging, and molecular chaperone genes. (A) The number of genes for gene types involved in sexual reproduction or thermal tolerance in the SM and MI transcriptomes. The number of DEGs at 32 °C from each gene type (Trinity/edgeR: fold ≥ 4 and FDR ≤ 0.001 relative to 27 °C) are shown for each population on (B) day 9 and (C) day 13. Gene types that had no DEGs in either population are excluded from (B) and (C). Gene abbreviations are as follows: mutS protein homolog 4 (Msh4), mutS protein homolog 5 (Msh5), meiotic recombination protein Spo11-2 (Spo11-2), copper/zinc superoxide dismutase (Cu/Zn-Sod), iron superoxide dismutase (Fe-Sod), manganese superoxide dismutase (Mn-Sod), nickel superoxide dismutase (Ni-Sod), catalase-peroxidase (KatG), ascorbate peroxidase (Apx), cytochrome c peroxidase (Ccpr), glutathione peroxidase (Gpx), peroxiredoxin (Prdx), heat shock protein 70 (Hsp70), heat shock protein 90 (Hsp90). Additional genes are shown in supplementary fig. S2, Supplementary Material online. DEG annotation and differential expression details are provided in supplementary tables S4–S7, Supplementary Material online.