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PCR gene amplification conditions for GAPDH, osteopontin, osteonectin, PPARγ, and C/EBPA gene for adipogenesis and osteogenesis differentiation assays.
| Process | Temperature (°C) | Time | Cycle |
|---|---|---|---|
| Predenaturation | 95 | 3 min | 1 |
| Denaturation | 95 | 30 sec | 35 |
| Annealing | 1 min | 35 | |
| GAPDH | 48.1 | ||
| Osteopontin | 57.8 | ||
| Osteonectin | 60.5 | ||
| PPARγ | 47.3 | ||
| C/EBPA | 66.7 | ||
| Elongation | 72 | 1 min | 35 |
| Final elongation | 72 | 10 min | 1 |
| Incubation | 10 | ∞ | ∞ |
