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. 2016 Aug 18;6:31914. doi: 10.1038/srep31914

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The concentration of symplasmata was measured after 24 hours of incubation in liquid medium. Error bars represent one standard deviation calculated from 11 replicates (wild-type P. eucalypti 299R), duplicates (cspD::Tn5), or triplicates (all other strains). Inserted micrographs show the phenotype of mutants lrhA::Tn5, rfbX3::Tn5, and cspD::Tn5 (note: phzI::Tn5 and gshA::Tn5 are not shown as they looked identical to lrhA::Tn5). Bars are 10 μm.