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. 2016 Aug 18;6:31899. doi: 10.1038/srep31899

Table 1. Kinetic parameters of the SrtA variants.

Mutants Mutations Kcat, s−1 KmLPETG, mM Kcat/KmLPETG, M−1 s−1 KmGGG, μM
WT   1.1 ± 0.1 7.1 ± 0.9 159 ± 25 71 ± 8
5M P94R/D160N/D165A/K190E/K196T 3.7 ± 0.2 1.0 ± 0.1 3622 ± 537 2779 ± 610
5M/D124G P94R/D124G/D160N/D165A/K190E/K196T 16.5 ± 0.7 1.8 ± 0.2 9305 ± 931 2833 ± 742
D124G D124G 1.8 ± 0.2 3.9 ± 0.7 472 ± 99 247 ± 40
5M/Y187R/E189K P94R/D160N/D165A/Y187R/E189K/K190E/K196T 5.2 ± 0.6 2.8 ± 0.5 1868 ± 400 1412 ± 138
5M/D124G//Y187R/E189K P94R/D124G/D160N/D165A/Y187R/E189K/K190E/K196T 7.9 ± 0.6 1.4 ± 0.2 5519 ± 901 1981 ± 316
5M/Y187L/E189A P94R/D160N/D165A/Y187L/E189A/K190E/K196T 2.9 ± 0.2 0.7 ± 0.1 4094 ± 748 5580 ± 1204
5M/D124G//Y187L/E189A P94R/D124G/D160N/D165A/Y187L/E189A/K190E/K196T 15.9 ± 1.7 1.5 ± 0.3 10405 ± 2451 2004 ± 265
5M/Y187L/E189R P94R/D160N/D165A/Y187L/E189R/K190E/K196T 6.3 ± 0.3 0.55 ± 0.06 11425 ± 1315 2367 ± 610
5M/D124G//Y187L/E189R P94R/D124G/D160N/D165A/Y187L/E189R/K190E/K196T 22.2 ± 1.3 1.3 ± 0.2 16722 ± 2329 1700 ± 420