Table 1. Kinetic parameters of the SrtA variants.
Mutants | Mutations | Kcat, s−1 | KmLPETG, mM | Kcat/KmLPETG, M−1 s−1 | KmGGG, μM |
---|---|---|---|---|---|
WT | 1.1 ± 0.1 | 7.1 ± 0.9 | 159 ± 25 | 71 ± 8 | |
5M | P94R/D160N/D165A/K190E/K196T | 3.7 ± 0.2 | 1.0 ± 0.1 | 3622 ± 537 | 2779 ± 610 |
5M/D124G | P94R/D124G/D160N/D165A/K190E/K196T | 16.5 ± 0.7 | 1.8 ± 0.2 | 9305 ± 931 | 2833 ± 742 |
D124G | D124G | 1.8 ± 0.2 | 3.9 ± 0.7 | 472 ± 99 | 247 ± 40 |
5M/Y187R/E189K | P94R/D160N/D165A/Y187R/E189K/K190E/K196T | 5.2 ± 0.6 | 2.8 ± 0.5 | 1868 ± 400 | 1412 ± 138 |
5M/D124G//Y187R/E189K | P94R/D124G/D160N/D165A/Y187R/E189K/K190E/K196T | 7.9 ± 0.6 | 1.4 ± 0.2 | 5519 ± 901 | 1981 ± 316 |
5M/Y187L/E189A | P94R/D160N/D165A/Y187L/E189A/K190E/K196T | 2.9 ± 0.2 | 0.7 ± 0.1 | 4094 ± 748 | 5580 ± 1204 |
5M/D124G//Y187L/E189A | P94R/D124G/D160N/D165A/Y187L/E189A/K190E/K196T | 15.9 ± 1.7 | 1.5 ± 0.3 | 10405 ± 2451 | 2004 ± 265 |
5M/Y187L/E189R | P94R/D160N/D165A/Y187L/E189R/K190E/K196T | 6.3 ± 0.3 | 0.55 ± 0.06 | 11425 ± 1315 | 2367 ± 610 |
5M/D124G//Y187L/E189R | P94R/D124G/D160N/D165A/Y187L/E189R/K190E/K196T | 22.2 ± 1.3 | 1.3 ± 0.2 | 16722 ± 2329 | 1700 ± 420 |