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. 2016 Aug 18;6:31869. doi: 10.1038/srep31869

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Copyright © 2016, The Author(s)

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(a) Schematic representation of experimental procedure is shown. Vesicles were isolated from independent pools of rat sera by either addition of TEI or by ultracentrifugation for 2 (standard) or 18 (extended) hours. Following NTA was used to measure residual vesicles in the supernatant, compared to the number of vesicles present in the whole sera (Not Centrifuged). Measurements from two independent pools are shown. (b) Residual EVs distribution and (c) Number of residual EVs as measured by NTA. N.D. Non-detectable.