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. 2016 Aug 18;6:31869. doi: 10.1038/srep31869

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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To assess whether the vesicles isolated via TEI-induced precipitation were active, freshly isolated rat primary were incubated over night with 1.9 × 10¹² vesicles, which were either stained with Syto RNAselect (left panel) or left unstained (right panel). Vesicle uptake was recorded by live imaging.