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. 2016 Aug 18;6:31869. doi: 10.1038/srep31869

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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RNAs isolated from whole sera (TOTAL), from vesicles pelleted by either Total Exosome Isolation reagent-based (TEI) or Standard ultracentrifugation (ULTRA) were reverse transcribed following the miQPCR protocol, and the expression of a panel of miRNAs was analyzed by individual qPCR assays. qPCR data were median normalized as described in ref. 52. Data are represented as average ± standard deviation calculated from four independent isolations (n = 4). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.0001; n.s., non-significant.