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. 2016 Aug 18;6:31869. doi: 10.1038/srep31869

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Sera isolated from rats recovering from PHx were subjected to centrifugation (20000 rcf/20 min). EVs present in the clarified supernatant were measured by using NTA. Data are represented as average ± standard deviation. Statistical analysis was performed by unpaired T-test (day 0; n = 3) versus individual regeneration groups (day 2, 4 and 6 after PHx; n = 3 within each group). *P ≤ 0.05.