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. 2016 Aug 18;6:31869. doi: 10.1038/srep31869

Table 1. Sequences of primers used for measuring miRNA expression by miQPCR.

Name Sequences 5' > 3'
rno-Let-7a AGGTAGTAGGTTGTATAGTTG
rno-miR-122 GAGTGTGACAATGGTGTTTGG
rno-miR-125 TGAGGTTCTTGGGAGCCG
rno-miR-142-3p TGTAGTGTTTCCTACTTTATGGAG
rno-miR-143 AGATGAAGCACTGTAGCTCAG
rno-miR-150 CCAACCCTTGTACCAGTGG
rno-miR-155 TTAATGCTAATTGTGATAGGGGTG
rno-miR-15a TAGCAGCACATAATGGTTTGTGG
rno-miR-15b TAGCAGCACATCATGGTTTACAG
rno-miR-16 CAGCACGTAAATATTGGCGG
rno-miR-18a GGTGCATCTAGTGCAGATAGG
rno-miR-192 TGACCTATGAATTGACAGCCG
rno-miR-194 GTAACAGCAACTCCATGTGGAG
rno-miR-21 TAGCTTATCAGACTGATGTTGAGG
rno-miR-223 TCAGTTTGTCAAATACCCCAG
rno-miR-30d TCCCCGACTGGAAGG
rno-miR-33 TGCATTGTAGTTGCATTGCAG
rno-miR-451 AACCGTTACCATTACTGAGTTGG
rno-miR-92a CTTGTCCCGGCCTGG
rno-miR-98 AGGTAGTAAGTTGTATTGTTGG
RNU6 GCAAGGATGACACGCAAATT
Upm2A CCCAGTTATGGCCGTTTA

Upm2A indicates the sequence of the universal primer used for qPCR, while MQ-RT indicates the sequence of the universal reverse transcription primer. miRNA-primers included in this analysis were selected with respect to their expression in the liver (miR-12255, miR-19455 and miR-19255), their function during infection, inflammation (miR-15556), or regulation of HSCs function (miR-15043), or their increased expression during liver regeneration (miR-3357, miR-2158). Whereas, other miRNAs and small non-coding RNAs included in the panel were selected for being ubiquitously expressed (RNU655 and Let-7a55), for being enriched in brain (miR-14355,59) or for being either associated to hematopoiesis (miR-45160) or hepatoblastoma (miR-12561).