Table 1. Sequences of primers used for measuring miRNA expression by miQPCR.
Name | Sequences 5' > 3' |
---|---|
rno-Let-7a | AGGTAGTAGGTTGTATAGTTG |
rno-miR-122 | GAGTGTGACAATGGTGTTTGG |
rno-miR-125 | TGAGGTTCTTGGGAGCCG |
rno-miR-142-3p | TGTAGTGTTTCCTACTTTATGGAG |
rno-miR-143 | AGATGAAGCACTGTAGCTCAG |
rno-miR-150 | CCAACCCTTGTACCAGTGG |
rno-miR-155 | TTAATGCTAATTGTGATAGGGGTG |
rno-miR-15a | TAGCAGCACATAATGGTTTGTGG |
rno-miR-15b | TAGCAGCACATCATGGTTTACAG |
rno-miR-16 | CAGCACGTAAATATTGGCGG |
rno-miR-18a | GGTGCATCTAGTGCAGATAGG |
rno-miR-192 | TGACCTATGAATTGACAGCCG |
rno-miR-194 | GTAACAGCAACTCCATGTGGAG |
rno-miR-21 | TAGCTTATCAGACTGATGTTGAGG |
rno-miR-223 | TCAGTTTGTCAAATACCCCAG |
rno-miR-30d | TCCCCGACTGGAAGG |
rno-miR-33 | TGCATTGTAGTTGCATTGCAG |
rno-miR-451 | AACCGTTACCATTACTGAGTTGG |
rno-miR-92a | CTTGTCCCGGCCTGG |
rno-miR-98 | AGGTAGTAAGTTGTATTGTTGG |
RNU6 | GCAAGGATGACACGCAAATT |
Upm2A | CCCAGTTATGGCCGTTTA |
Upm2A indicates the sequence of the universal primer used for qPCR, while MQ-RT indicates the sequence of the universal reverse transcription primer. miRNA-primers included in this analysis were selected with respect to their expression in the liver (miR-12255, miR-19455 and miR-19255), their function during infection, inflammation (miR-15556), or regulation of HSCs function (miR-15043), or their increased expression during liver regeneration (miR-3357, miR-2158). Whereas, other miRNAs and small non-coding RNAs included in the panel were selected for being ubiquitously expressed (RNU655 and Let-7a55), for being enriched in brain (miR-14355,59) or for being either associated to hematopoiesis (miR-45160) or hepatoblastoma (miR-12561).