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. 2016 Aug 18;6:32021. doi: 10.1038/srep32021

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) The CARS images of N2, PUFA-deficient mutants (fat-1, fat-2, fat-3, and fat-4), and fat-2 worms supplemented with PUFAs. Red arrows indicate the regions of yolk lipoprotein accumulation. E stands for embryo, I stands for intestine, and V stands for vulva. Scale bar = 30 μm. (b) The total area and (c) the average CARS intensity of yolk lipoprotein accumulations in the pseudocoelomic cavity of uterus in different worms. (n = 57 for N2, and n = 19~32 for mutants and PUFA-supplemented fat-2 worms) All worms are 1D-Ad worms. Error bars represent the SEM. (d–k) The area contribution curves of yolk lipoprotein accumulations in various strains. (P-value: *p < 0.05, **p < 0.01, ***p < 0.001, ns: not significant).