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. 2016 Aug 18;6:31694. doi: 10.1038/srep31694

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) CP-A cells in Oris™ migration wells were labeled with CellTrace Violet and the pre-migration, t₀ image was captured (left panel). Cells were allowed to migrate for 14 hours, then the final image, t_f, was captured (right panel). (b) Two regions of interest (ROIs) were created on the t₀ image (left). ROI-1 (red) includes all cells, ROI-2 (green) delineate the edge of the cell-free exclusion zone. Both ROIs are pasted, maintaining their relative postions, onto the post-migration, t_f, image (right). Migration is assessed as the percent of flourescent signal in ROI-2 relative to total fluorescence (ROI-1).