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. 2016 Aug 18;6:31694. doi: 10.1038/srep31694

Figure 2. CellTrace dyes do not alter cell migration or proliferation.

Figure 2

(a) EPC2 (EPC), CP-A, HeLa, and MDA-MB-231 (MDA231) cells were seeded into migration wells and labeled with various concentrations of CFR before migration. After migration, cells were labeled with the nucleic acid stain SYTO 24 and imaged. NIS-Elements was used to quantify the number of nuclei in the exclusion zone. Data were pooled from two independent experiments each consisting of four replicates. CFR treatment had no discernible effect on migration (p = 0.99). (b) Similar to (a) except cells were labeled with the indicated concentrations of CV during migration. This treatment had no discernible effect on migration (p = 0.87) (c) Cells were seeded subconfluently into a 96-well plated without migration barriers. Cells were exposed to CFR at the indicated concentrations for 24 hours. Then cells were labeled with SYTO 24 and total cells were enumerated using NIS-Elements. CFR had no discernible effect on cell numbers after 24 hours (p = 0.96). (d) Similar to (c) except cells were exposed to the indicated concentrations of CV. This treatment had no measurable effect on cell numbers (p = 0.80). Error bars indicate SEM. Data were analyzed by two-way ANOVA with Dunnett’s multiple comparisons test.