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. 2016 Aug 18;6:31694. doi: 10.1038/srep31694

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) 14 hour migration assays of EPC2 (EPC), CP-A (CPA), HeLa, and Swiss 3T3 (3T3) cells that were exposed to 1 μg/mL cytochalasin D (cyto D) during migration, or pretreated for 2 hours with 10 μg/mL mitomycin C (MMC) immediately prior to migration. (b) Migration results pooled from three independent experiments as in (a). Cytochalasin D significantly inhibited migration in all cell types, while mitomycin C inhibited migration of EPC2 and CP-A cells but not HeLa or Swiss 3T3 cells. (c) A panel of human breast epithelial cell lines, HME1 (HME), MCF 10A, MCF7, and MDA-MB-231(MDA) were treated with cytochalasin D (cyto D), or mitomycin C (MMC). (d) Migration results pooled from three independent experiments as in (c). Under control conditions, MCF7 migration was significantly lower than both MCF 10A and MDA-MB-231. Cytochalasin D treatment inhibited migration in all cell lines except MCF7, and mitomycin C had no effect on any cell line. Data in (b,d) present the means of three independent experiments each performed in duplicate and error bars indicate SEM. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. Data were analyzed by two-way ANOVA with Tukey’s multiple comparisons test.