Figure 2. Antiviral activity of GPI-modified TM-CC(aT) and PV1 proteins.
(A) Infectious virion yield measured using HeLa TZM-bl indicator cells following transfection with a Vpu-deficient HIV-1 proviral plasmid along with increasing amounts of the indicated unmodified (WT) or GPI-modified (+GPI) Tetherin, TM-CC(aT) or PV1 proteins (RLU= relative light units, mean ± SD, n=3).
(B) Western blot analyses (anti-CA) of cell lysates and virions corresponding to (A). Numbers at the bottom represent virion CA protein levels relative to those obtained in the absence of an inhibitor.
See also Figure S2.