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. 2016 Mar 8;10(9):2129–2144. doi: 10.1038/ismej.2016.23

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Copyright © 2016 International Society for Microbial Ecology

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Discovery of a minimal Euo target box. (a) Several minimal Euo boxes (green) are present in P_queF, P_ftsY and P_ndh. Euo consensus binding site is also represented in light green. (b) Consensus based on these Euo boxes showing the conservation of the TTT. The TTT was replaced by GGG (shown in red) and the His₆-Euo binding was tested by EMSA (c–e). The synthetic DNAs carrying the mutations were amplified using a specific primer coupled to Cy5 and used for EMSA. Mutation (red boxes) of the four Euo boxes (green) in P_queF and P_ndh completely abolished the binding of His₆-Euo as no band-shift was observed (c, d). Mutations in the two first Euo boxes in P_ftsY completely abolished the binding of His₆-Euo (e).