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. 2016 Mar 8;10(9):2280–2292. doi: 10.1038/ismej.2016.9

Table 1. Overview of number of samples, number of 16S rRNA gene sequences retrieved and corresponding diversity indices (average±s.d.) for each sampling group within two experiments (natural dynamics and disturbance experiment).

Sampling group Total no. of samples No. of sequencesa Richness Evenness Shannon index OTUs in totala Chao estimatea
Natural dynamics
 New mucus 14 4882±2534 50±20 0.49±0.19 1.95±0.38 174 256
 Aged mucus 6 5867±2770 82±29 0.81±0.05 3.48±0.90 178 248
 Seawater 6 7709±3758 28±8 0.59±0.15 2.07±0.52 66 88
 Sediment 4 923±116 70±12 0.80±0.05 3.40±0.30 137 210
               
Disturbance experiment
 Initial composition 12 5941±2962 54±12 0.55±0.14 2.23±0.72 170 244
 Aquaria treated 6 3837±2436 50±12 0.75±0.07 2.33±0.45 109 155
 Aquaria control 6 4122±3249 84±19 0.81±0.05 3.32±0.47 176 230
 1–3 Days treatedb 18 7000±3728 67±17 0.68±0.10 2.84±0.54 201 316
 1–3 Days controlb 18 5494±1563 81±18 0.71±0.11 3.42±0.34 231 309
 4–28 Days treatedb 10 5528±3499 46±20 0.70±0.13 2.64±0.67 138 186
 4–28 Days controlb 10 8003±2583 71±15 0.78±0.06 3.17±0.43 183 315

All data are based on a rarefied OTU table at the family level from which chloroplast-affiliated OTUs were removed.

Abbreviations: OTU, operational taxonomic unit; rRNA, ribosomal RNA.

a

‘No. of sequences', ‘OTUs in total' and ‘Chao estimate' were calculated from the original non-rarefied OTU data set (including chloroplasts).

b

1–3 and 4–28 days represent the number of days after re-introduction on the reef. Data were pooled for each of those two periods.