Table 1. Overview of number of samples, number of 16S rRNA gene sequences retrieved and corresponding diversity indices (average±s.d.) for each sampling group within two experiments (natural dynamics and disturbance experiment).
| Sampling group | Total no. of samples | No. of sequencesa | Richness | Evenness | Shannon index | OTUs in totala | Chao estimatea |
|---|---|---|---|---|---|---|---|
| Natural dynamics | |||||||
| New mucus | 14 | 4882±2534 | 50±20 | 0.49±0.19 | 1.95±0.38 | 174 | 256 |
| Aged mucus | 6 | 5867±2770 | 82±29 | 0.81±0.05 | 3.48±0.90 | 178 | 248 |
| Seawater | 6 | 7709±3758 | 28±8 | 0.59±0.15 | 2.07±0.52 | 66 | 88 |
| Sediment | 4 | 923±116 | 70±12 | 0.80±0.05 | 3.40±0.30 | 137 | 210 |
| Disturbance experiment | |||||||
| Initial composition | 12 | 5941±2962 | 54±12 | 0.55±0.14 | 2.23±0.72 | 170 | 244 |
| Aquaria treated | 6 | 3837±2436 | 50±12 | 0.75±0.07 | 2.33±0.45 | 109 | 155 |
| Aquaria control | 6 | 4122±3249 | 84±19 | 0.81±0.05 | 3.32±0.47 | 176 | 230 |
| 1–3 Days treatedb | 18 | 7000±3728 | 67±17 | 0.68±0.10 | 2.84±0.54 | 201 | 316 |
| 1–3 Days controlb | 18 | 5494±1563 | 81±18 | 0.71±0.11 | 3.42±0.34 | 231 | 309 |
| 4–28 Days treatedb | 10 | 5528±3499 | 46±20 | 0.70±0.13 | 2.64±0.67 | 138 | 186 |
| 4–28 Days controlb | 10 | 8003±2583 | 71±15 | 0.78±0.06 | 3.17±0.43 | 183 | 315 |
All data are based on a rarefied OTU table at the family level from which chloroplast-affiliated OTUs were removed.
Abbreviations: OTU, operational taxonomic unit; rRNA, ribosomal RNA.
‘No. of sequences', ‘OTUs in total' and ‘Chao estimate' were calculated from the original non-rarefied OTU data set (including chloroplasts).
1–3 and 4–28 days represent the number of days after re-introduction on the reef. Data were pooled for each of those two periods.