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. 2016 Aug 17;9:456. doi: 10.1186/s13071-016-1741-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — SDS-PAGE and western blotting analysis of Eg-Grx1. Lane M, molecular weight marker; Lane 1, IPTG-induced E. coli BL21 (DE3) lysate from cells expressing rEg-Grx1; Lane 2, purified rEg-Grx1; Lane 3, purified rEg-Grx1 probed with anti-rEg-Grx1 rabbit serum; Lane 4, purified rEg-Grx1 probed with native (pre-immune) rabbit serum; Lane 5, purified rEg-Grx1 probed with the serum of E. granulosus infected sheep; Lane 6, purified rEg-Grx1 probed with native (healthy) sheep serum; Lane 7, the total protein from protoscoleces probed with anti-Eg-Grx1 rabbit serum; Lane 8, the total protein from protoscoleces probed with native (pre-immune) rabbit serum