Figure 2.

Nucleotide alignments of Gasterosteus aculeatus and Pungitius pungitius interchromosomal spiggin chimerics with the 2006 draft (Broad/gasAcu1) assembly of G. aculeatus and parental genes. Alignments of the G. aculeatus (A) and P. pungitius (B) interchromosomal spiggin chimerics with spiggin B (AB910011) and the recruited region of the draft assembly. The spiggin region of the P. pungitius interchromosomal chimeric has not been annotated with a specific spiggin gene as the spiggin multigene family in P. pungitius has not been fully characterized. Putatively assigned introns are indicated in grey. Black indicates nucleotide differences between sequences within the alignment. Black triangles represent start codons, grey triangles represent stop codons, and the white triangles indicate polyadenylation signals (AAUAAA). Chr, chromosome.