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. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — KLF8 mediates mesendoderm to DE differentiation. a Schematics of siRNA knockdown strategy. FACS analysis of CXCR4 and T-2A-EGFP expression at day two of differentiation with representative gene-specific siRNA transfection results. b Summary Differentiation Scores of siRNA knockdown experiments of the top 25 genes tested. The dashed line indicates a Differentiation Score of 1, set by the non-target siRNA control. Genes in blue font indicate control experiments. c Upper panel, schematics of KLF8 overexpression strategy during differentiation. Lower panel, FACS analysis shows CXCR4 and T-2A-EGFP expression at day two of differentiation. DOX Doxycycline. d QPCR analysis of three independent KLF8 overexpression clones tested at day two of differentiation. All data are shown as mean ± S.D. ***p <0.001; **p <0.01; *p <0.05, all by one-tailed t-test. In all FACS plots, the x-axis indicates GFP/FITC channel, the y-axis indicates APC channel