Fig. 6.

a-k Venom Heatmap for A. alata. Hierarchical clustering (EdgeR) and corresponding ten subcluster profiles for the 455 genes implicated in venom differentially expressed across A. alata medusa (gastric cirri, ovaries, tentacle (with pedalium base), rhopalium) and planulae samples. Intensity of color indicates expression levels for each of the ten hierarchical clusters (vertical access). Bright yellow patches correspond to the highest peaks for each k-mean subcluster profile. K-mean profiles (b-k) match the order of column names in a, representing the mean expression of gene clusters highly abundant in each sample (centroid demarcated by the solid line; zero indicated by the horizontal dashed red line). Two bright yellow transcript clusters in the gastric cirri column correspond to peaks in plots b and c; one cluster in the ovaries column corresponds to plot d; four clusters in the tentacle column correspond to plots e, f, g and h; one cluster in the rhopalium corresponds to plot j and to the less prominent peak seen in plot i; one cluster in the planulae column corresponds to plot k. The vertical colored bar on the left of the heatmap (a) indicates distinct patterns corresponding to the ten subcluster profiles (sc = subcluster number), for which the number of genes each comprises is indicated. Abbreviations: gc = gastric cirri, ov = ovaries, tnt = tentacle (and pedalium base), rhp = rhopalium, and pln = planulae. Gene annotations by subcluster provided in Additional file 7