Table 1.
Whole transcriptome | Filtered transcriptome (fpkm = 1.5) | |
---|---|---|
No. of transcripts | 126,484 | 31,776 |
No. of genes | 84,124 | 20,173 |
Total assembled bases (bp) | 125,647,941 | 48,556,932 |
Avg (mean) transcript length (bp) | 993 | 1528 |
Median transcript length (bp) | 456 | 989 |
Max transcript length (bp) | 9993 | 9993 |
N50 | 1994 | 2545 |
GC content (%) | 39 | 40 |
Percent proper pairs | 77 | 78 |
Samtools percent mapped and paired | 75 | 77 |
De novo assembly began with 264,505,922 trimmed raw reads generated from medusa gastric cirri, ovaries, tentacle (with pedalium base), rhopalium, and planulae samples