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Western blotting to examine modulation of expression of molecules involved in induction of apoptosis in human Ewing’s sarcoma SK-N-MC and RD-ES cell lines. Treatments: scrambled shRNA plasmid (0.5 μg/ml) transfection for 72 h, EWS shRNA plasmid (0.5 μg/ml) transfection for 72 h, 100 μM TFL treatment for 24 h, and EWS shRNA palsmid (0.5 μg/ml) transfection for 48 h + 100 μM TFL treatment for last 24 h.
