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. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: J Cancer Stem Cell Res. 2014 Dec 1;2:e1008. doi: 10.14343/JCSCR.2014.2e1008

Figure 1. Strategy for Generation of 293T Cells Producing Lentivirus in Cancer Stem Cell Media.

Figure 1

Diagram representing the methods used to isolate a subline of LentiX-293Ts capable of growing and producing virus in cancer stem cell media. LentiX-293Ts were initially cultured in a 1:1 mixture of the 293T standard media (DMEM +10% FBS) and cancer stem cell media (DMEM/F12+EGF+FGF+B27). Once LentiX-293Ts grew to confluence, they were passaged into cancer stem cell media on geltrex coated dishes. Cells were then continually passaged in cancer stem cell media on geltrex to obtain a LentiX-293T subline that proliferated in the absence of serum, designated CSC293T. We anticipate that a similar strategy can be used to develop 293T sublines for producing lentivirus in many other medias required for specific cell types.