Table 1.
Distribution of total variants (a) and nonsynonymous mutations (b) in analyzed samples.
| (a) | Total variants |
AMER1 | APC | ARID1A | BRAF | FBXW7 | KRAS | MSH3 | MSH6 | NRAS | PIK3CA | SMAD4 | SOX9 | TCF7L2 | TGFBR2 | TP53 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Illumina | CC1029-AA-CRC-FF | 55 | 5 | 10 | 0 | 2 | 2 | 10 | 7 | 3 | 1 | 3 | 1 | 3 | 2 | 3 | 3 |
| Illumina | CC1029-AA-CRC-FF | 56 | 4 | 8 | 2 | 2 | 1 | 8 | 8 | 3 | 2 | 5 | 1 | 2 | 2 | 4 | 4 |
| Illumina | CC1029-AA-Normal-FF | 59 | 5 | 9 | 0 | 3 | 1 | 11 | 7 | 2 | 4 | 4 | 2 | 4 | 2 | 2 | 3 |
| Illumina | CC1029-AA-Normal-FF | 52 | 4 | 7 | 1 | 2 | 1 | 8 | 7 | 2 | 2 | 4 | 4 | 2 | 2 | 2 | 4 |
| Illumina | CC1053-AA-Normal-FFPE | 44 | 9 | 5 | 1 | 2 | 0 | 4 | 4 | 3 | 2 | 5 | 0 | 4 | 1 | 2 | 2 |
| Illumina | CC1053-AA-Normal-FF | 46 | 5 | 5 | 1 | 2 | 2 | 3 | 3 | 4 | 3 | 6 | 0 | 4 | 2 | 3 | 3 |
| Ion Torrent | CC1053-AA-Normal-FFPE | 46 | 3 | 3 | 5 | 3 | 2 | 3 | 5 | 6 | 1 | 8 | 0 | 2 | 2 | 1 | 2 |
| Ion Torrent | CC1053-AA-Normal-FF | 39 | 2 | 3 | 1 | 3 | 2 | 3 | 4 | 6 | 1 | 8 | 0 | 1 | 1 | 1 | 3 |
| Illumina | CC1054-AA-CRC-FFPE | 85 | 12 | 11 | 4 | 3 | 1 | 11 | 9 | 5 | 1 | 5 | 3 | 2 | 9 | 5 | 4 |
| Illumina | CC1054-AA-CRC-FF | 60 | 6 | 11 | 1 | 3 | 0 | 7 | 9 | 4 | 0 | 5 | 2 | 2 | 3 | 4 | 3 |
| Ion Torrent | CC1054-AA-CRC-FFPE | 283 | 33 | 26 | 28 | 18 | 9 | 7 | 26 | 27 | 2 | 24 | 11 | 17 | 22 | 4 | 29 |
| Ion Torrent | CC1054-AA-CRC-FF | 86 | 3 | 15 | 7 | 7 | 3 | 6 | 14 | 12 | 1 | 9 | 2 | 1 | 1 | 1 | 4 |
| Illumina | CC1057-AA-Normal-FFPE | 42 | 6 | 1 | 2 | 2 | 1 | 5 | 7 | 4 | 2 | 3 | 0 | 3 | 1 | 3 | 2 |
| Illumina | CC1057-AA-Normal-FF | 48 | 2 | 12 | 0 | 2 | 3 | 5 | 2 | 5 | 2 | 5 | 1 | 2 | 1 | 3 | 3 |
| Ion Torrent | CC1057-AA-Normal-FFPE | 50 | 3 | 1 | 1 | 11 | 2 | 3 | 13 | 6 | 1 | 3 | 0 | 2 | 1 | 1 | 2 |
| Ion Torrent | CC1057-AA-Normal-FF | 55 | 1 | 12 | 0 | 4 | 2 | 3 | 5 | 10 | 1 | 7 | 2 | 0 | 2 | 3 | 3 |
| Ion Torrent | CC1057-AA-Normal-FF | 57 | 1 | 12 | 1 | 2 | 6 | 2 | 3 | 15 | 0 | 5 | 2 | 0 | 2 | 3 | 3 |
| Ion Torrent | CC1057-AA-CRC-FF | 52 | 1 | 10 | 0 | 5 | 2 | 3 | 5 | 9 | 1 | 7 | 2 | 0 | 2 | 3 | 2 |
| Ion Torrent | CC1057-AA-CRC-FF | 58 | 1 | 12 | 1 | 5 | 2 | 3 | 5 | 10 | 1 | 7 | 2 | 0 | 2 | 4 | 3 |
| Illumina | CC1059-AA-CRC-FF | 42 | 5 | 3 | 1 | 5 | 0 | 4 | 8 | 5 | 2 | 3 | 0 | 2 | 2 | 1 | 1 |
| Illumina | CC1059-AA-CRC-FF | 40 | 5 | 1 | 1 | 3 | 1 | 3 | 8 | 5 | 2 | 4 | 0 | 2 | 3 | 1 | 1 |
| Ion Torrent | CC1059-AA-CRC-FF | 49 | 2 | 4 | 3 | 4 | 1 | 3 | 14 | 11 | 1 | 2 | 0 | 1 | 0 | 1 | 2 |
| Ion Torrent | CC1059-AA-CRC-FF | 49 | 3 | 4 | 3 | 4 | 1 | 3 | 13 | 11 | 1 | 2 | 0 | 1 | 0 | 2 | 1 |
| Ion Torrent | CC1059-AA-Normal-FF | 48 | 2 | 4 | 3 | 4 | 1 | 3 | 16 | 10 | 1 | 2 | 0 | 1 | 0 | 1 | 0 |
| Ion Torrent | CC1059-AA-Normal-FF | 43 | 2 | 4 | 1 | 4 | 1 | 3 | 12 | 11 | 1 | 2 | 0 | 1 | 0 | 1 | 0 |
| Illumina | CC1060-AA-CRC-FF | 52 | 5 | 8 | 0 | 6 | 1 | 10 | 4 | 2 | 2 | 3 | 2 | 4 | 0 | 2 | 3 |
| Illumina | CC1060-AA-CRC-FF | 49 | 5 | 7 | 1 | 4 | 1 | 7 | 4 | 2 | 3 | 2 | 3 | 5 | 0 | 2 | 3 |
| Illumina | CC1060-AA-Normal-FF | 52 | 6 | 7 | 1 | 6 | 2 | 8 | 5 | 3 | 1 | 3 | 2 | 4 | 0 | 2 | 2 |
| Illumina | CC1060-AA-Normal-FF | 43 | 6 | 6 | 0 | 3 | 1 | 7 | 3 | 2 | 2 | 3 | 2 | 3 | 1 | 2 | 2 |
| non-synonymous | |||||||||||||||||
| (b) | Total variants |
AMER1 | APC | ARID1A | BRAF | FBXW7 | KRAS | MSH3 | MSH6 | NRAS | PIK3CA | SMAD4 | SOX9 | TCF7L2 | TGFBR2 | TP53 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Illumina | CC1029-AA-CRC-FF | 48 | 5 | 4 | 0 | 1 | 2 | 10 | 7 | 3 | 1 | 3 | 1 | 3 | 2 | 3 | 3 |
| Illumina | CC1029-AA-CRC-FF | 48 | 3 | 3 | 2 | 1 | 1 | 8 | 7 | 3 | 2 | 5 | 1 | 2 | 2 | 4 | 4 |
| Illumina | CC1029-AA-Normal-FF | 48 | 4 | 4 | 0 | 2 | 1 | 10 | 6 | 2 | 2 | 4 | 2 | 4 | 2 | 2 | 3 |
| Illumina | CC1029-AA-Normal-FF | 45 | 3 | 3 | 1 | 1 | 1 | 8 | 6 | 2 | 2 | 4 | 4 | 2 | 2 | 2 | 4 |
| Illumina | CC1053-AA-Normal-FFPE | 42 | 5 | 8 | 1 | 1 | 0 | 4 | 4 | 3 | 2 | 5 | 0 | 4 | 1 | 2 | 2 |
| Illumina | CC1053-AA-Normal-FF | 43 | 5 | 4 | 1 | 1 | 2 | 3 | 3 | 3 | 3 | 6 | 0 | 4 | 2 | 3 | 3 |
| Ion Torrent | CC1053-AA-Normal-FFPE | 38 | 3 | 2 | 2 | 2 | 2 | 2 | 5 | 5 | 1 | 8 | 0 | 1 | 2 | 1 | 2 |
| Ion Torrent | CC1053-AA-Normal-FF | 33 | 3 | 1 | 0 | 2 | 2 | 2 | 4 | 5 | 1 | 8 | 0 | 0 | 1 | 1 | 3 |
| Illumina | CC1054-AA-CRC-FFPE | 69 | 5 | 10 | 4 | 2 | 1 | 10 | 9 | 4 | 1 | 5 | 3 | 2 | 6 | 4 | 3 |
| Illumina | CC1054-AA-CRC-FF | 49 | 5 | 5 | 1 | 2 | 0 | 6 | 9 | 3 | 0 | 5 | 2 | 2 | 3 | 3 | 3 |
| Ion Torrent | CC1054-AA-CRC-FFPE | 217 | 13 | 22 | 19 | 17 | 7 | 5 | 24 | 21 | 2 | 20 | 9 | 9 | 20 | 2 | 27 |
| Ion Torrent | CC1054-AA-CRC-FF | 67 | 8 | 2 | 3 | 6 | 3 | 4 | 14 | 9 | 1 | 9 | 2 | 1 | 1 | 0 | 4 |
| Illumina | CC1057-AA-Normal-FFPE | 37 | 1 | 5 | 1 | 1 | 1 | 5 | 6 | 3 | 2 | 3 | 0 | 3 | 1 | 3 | 2 |
| Illumina | CC1057-AA-Normal-FF | 38 | 8 | 1 | 0 | 1 | 3 | 5 | 2 | 2 | 2 | 5 | 1 | 2 | 1 | 2 | 3 |
| Ion Torrent | CC1057-AA-Normal-FFPE | 44 | 1 | 2 | 1 | 10 | 2 | 2 | 12 | 4 | 1 | 3 | 0 | 2 | 1 | 1 | 2 |
| Ion Torrent | CC1057-AA-Normal-FF | 41 | 7 | 0 | 0 | 3 | 2 | 2 | 5 | 6 | 1 | 7 | 2 | 0 | 2 | 1 | 3 |
| Ion Torrent | CC1057-AA-Normal-FF | 41 | 7 | 0 | 1 | 1 | 5 | 1 | 3 | 10 | 0 | 5 | 2 | 0 | 2 | 1 | 3 |
| Ion Torrent | CC1057-AA-CRC-FF | 38 | 5 | 0 | 0 | 4 | 2 | 2 | 5 | 5 | 1 | 7 | 2 | 0 | 2 | 1 | 2 |
| Ion Torrent | CC1057-AA-CRC-FF | 44 | 7 | 0 | 1 | 4 | 2 | 2 | 5 | 6 | 1 | 7 | 2 | 0 | 2 | 2 | 3 |
| Illumina | CC1059-AA-CRC-FF | 36 | 2 | 4 | 0 | 4 | 0 | 4 | 8 | 4 | 2 | 3 | 0 | 1 | 2 | 1 | 1 |
| Illumina | CC1059-AA-CRC-FF | 34 | 1 | 4 | 0 | 2 | 1 | 3 | 8 | 3 | 2 | 4 | 0 | 1 | 3 | 1 | 1 |
| Ion Torrent | CC1059-AA-CRC-FF | 39 | 3 | 1 | 2 | 3 | 1 | 2 | 14 | 8 | 1 | 2 | 0 | 0 | 0 | 0 | 2 |
| Ion Torrent | CC1059-AA-CRC-FF | 39 | 3 | 2 | 2 | 3 | 1 | 2 | 13 | 8 | 1 | 2 | 0 | 0 | 0 | 1 | 1 |
| Ion Torrent | CC1059-AA-Normal-FF | 38 | 3 | 1 | 2 | 3 | 1 | 2 | 16 | 7 | 1 | 2 | 0 | 0 | 0 | 0 | 0 |
| Ion Torrent | CC1059-AA-Normal-FF | 33 | 3 | 1 | 0 | 3 | 1 | 2 | 12 | 8 | 1 | 2 | 0 | 0 | 0 | 0 | 0 |
| Illumina | CC1060-AA-CRC-FF | 42 | 3 | 4 | 0 | 5 | 1 | 10 | 3 | 2 | 1 | 3 | 2 | 3 | 0 | 2 | 3 |
| Illumina | CC1060-AA-CRC-FF | 41 | 3 | 4 | 1 | 3 | 1 | 7 | 4 | 2 | 2 | 2 | 3 | 4 | 0 | 2 | 3 |
| Illumina | CC1060-AA-Normal-FF | 43 | 3 | 5 | 0 | 5 | 2 | 8 | 4 | 3 | 1 | 3 | 2 | 3 | 0 | 2 | 2 |
| Illumina | CC1060-AA-Normal-FF | 36 | 2 | 5 | 0 | 2 | 1 | 7 | 2 | 2 | 2 | 3 | 2 | 3 | 1 | 2 | 2 |
| Analyzed Samples. | |||||||||||||||||