Extended Data Figure 6. Mpk1 regulates proteasome subunits and RACs post-transcriptionally.

a and b, Immunoblots of the indicated proteins in lysates of wild-type (WT) (a) and rpn4Δ (b) cells ± 5 µg/ml tunicamycin (Tm) or 0.2 µg/ml rapamycin (Rapa) for 4 hours. c, Immunoblots of the indicated proteins in lysates of WT cells carrying a TAP-tagged RPN4 at the endogenous locus ± 5 µg/ml tunicamycin (Tm) or 0.2 µg/ml rapamycin (Rapa) for 4 hours. d, Immunoblots of the indicated proteins in lysates of wild-type (WT) and mpk1Δ cells ± 5 µg/ml Tm or 0.2 µg/ml Rapa for 4 hours. e, rpn4Δ cells transformed with RPN4, MPK1, a kinase-dead allele of MPK1 (MPK1-K52R) or empty vector were spotted in a 6-fold dilution and grown on plates containing or lacking tunicamycin (Tm). f, mpk1Δ cells transformed with MPK1, RPN4 or empty vector were spotted in a 6-fold dilution and grown on plates containing or lacking tunicamycin (Tm) where indicated. g, Immunoblots of the indicated proteins in lysates of WT and mpk1Δ cells carrying a TAP-tagged RPN4 at the endogenous locus ± 5 µg/ml Tm or 0.2 µg/ml Rapa for 4 hours. h and i, Immunoblots of the indicated proteins in lysates of WT (h and i) and mpk1Δ (i) cells treated with different combinations of drugs: 5 µg/ml Tm, 0.2 µg/ml Rapa and 35 µg/ml cycloheximide (CHX), where indicated for 4 hours. Representative results of at least three independent experiments (biological replicates) are shown.