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. Author manuscript; available in PMC: 2017 Feb 11.
Published in final edited form as: Nature. 2016 Aug 11;536(7615):184–189. doi: 10.1038/nature18943

Extended Data Figure 9. TORC1 activation by nutrient replenishment decreases the abundance of RACs as well as 26S proteasome.

Extended Data Figure 9

a and b, Immunoblots (a) and quantification (b) of the indicated proteins in lysates of HeLa cells after replenishment with rich complete medium for the indicated time. c, Native-PAGE (4.2%) of cell extracts from HeLa cells following media replenishment as in (a), revealed with the fluorogenic substrate Suc-LLVY-AMC or by immunoblots. d, Quantification of the 26S proteasome activity (RPCP and RP2CP) of experiments such as the one shown in (c). b and d, Data are means ± SD. n=3 biological replicates. *P≤0.05; **P≤0.01; ***P≤0.001; n.s., not significant (one-way ANOVA). Representative results of at least three independent experiments (biological replicates) are shown.

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