Figure 2. The MAPK Mpk1 is a master regulator of the stress-inducible proteasome assembly chaperone Adc17.
a and b, Cells spotted in a 6-fold dilution and grown for 3 days on plates ± tunicamycin (Tm). c, Immunoblots from cells grown ± 5 µg/ml Tm for 4 hours. d and f, Cells transformed with empty vector or with MPK1 or HOG1 spotted in a 6-fold dilution and grown on plates ± 0.75 µg/ml Tm for 3 days. e and g, Immunoblots of lysates from cells grown ± 5 µg/ml Tm for 4 hours. h, Cartoon depicting the signalling pathway to Adc17.