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. Author manuscript; available in PMC: 2017 Feb 11.
Published in final edited form as: Nature. 2016 Aug 11;536(7615):184–189. doi: 10.1038/nature18943

Figure 3. Mpk1 coordinates the expression of all yeast RACs to control proteasome abundance.

Figure 3

a, Immunoblots of lysates from cells cultured ± 5 µg/ml tunicamycin (Tm) or 0.2 µg/ml rapamycin (Rapa) for 4 hours. b, Native-PAGE (4.2%) of yeast cells cultured ± 5 µg/ml Tm or 0.2 µg/ml Rapa, revealed with Suc-LLVY-AMC and by immunoblots. Rpt5i (Rpt5 intermediates). c and d, Quantifications from experiments as in (b). Data are means ± SD of four biological replicates. **P≤0.01; ***P≤0.001; n.s., not significant (two-way ANOVA). e, Immunoblots from lysates of cells cultured ± 5 µg/ml Tm or 0.2 µg/ml Rapa for 4 hours. f, Immunoblots from lysates of cells cultured at 30 or 37°C for 4 hours. g, As in (e).

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