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. Author manuscript; available in PMC: 2017 Feb 11.
Published in final edited form as: Nature. 2016 Aug 11;536(7615):184–189. doi: 10.1038/nature18943

Figure 5. Mpk1 adjusts proteasome degradation to match the needs.

Figure 5

a, Immunoblots of lysates of cells cultured ± 5 µg/ml tunicamycin (Tm) or 0.2 µg/ml rapamycin (Rapa) for 4 hours. Poly-Ub: polyubiquitinated conjugates. c and e, Immunoblots from lysates of cells expressing CPY*-HA (c) or Δss-CPY*-GFP (e) treated with 35 µg/ml cycloheximide (CHX) for the indicated time. (b, d, f) Quantification of (a, c, e), respectively. Data are means ± SD; n=4 (b) and n=3 (d and f) biological replicates.*P≤0.05; **P≤0.01; ***P≤0.001; n.s., not significant (two-way ANOVA).

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