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. Author manuscript; available in PMC: 2017 Feb 11.
Published in final edited form as: Nature. 2016 Aug 11;536(7615):184–189. doi: 10.1038/nature18943

Figure 6. Evolutionary conservation of the pathway controlling RACs and proteasome abundance.

Figure 6

a and b, Immunoblots (a) and quantifications (b) of the indicated proteins in lysates of HeLa cells treated with 250 nM Torin-1 for the indicated time. c and d, Native-PAGE (4.2%) (c) and quantifications (d) of HeLa cell lysates following treatment as in (a) and revealed with Suc-LLVY-AMC and by immunoblots. e, mpk1Δ cells transformed with a plasmid encoding the human Erk5 or an empty vector were spotted in a 6-fold dilution and grown on plates ± Tm for 3 days. f and g, Immunoblots (f) and quantifications (g) of the indicated proteins in lysates of HeLa cells 3 days after transfection with a non-target siRNA (siCTL) or a siRNA targeting Erk5 (siErk5). h and i, Native-PAGE (4.2%) (h) and quantifications (i) of HeLa cell extracts 3 days after transfection with siCTL or siErk5 revealed with Suc-LLVY-AMC or by immunoblots. (b, d, g, i) Data are means ± SD; n=3 biological replicates. *P≤0.05; **P≤0.01; ***P≤0.001; n.s., not significant (b and d, one-way ANOVA; g, two-way ANOVA; i, two-tailed Student’s t-test).

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