Extended Data Figure 3. Mpk1 MAPK pathway is essential for stress-mediated RACs induction.

a and b, Immunoblots of the indicated proteins in lysates of wild-type (WT) cells ± 5 µg/ml tunicamycin (Tm) (a) or 0.2 µg/ml rapamycin (Rapa) (b) for the indicated time. c and g, Immunoblots of the indicated proteins in lysates of WT and bck1Δ cells cultured ± 5 µg/ml Tm or 0.2 µg/ml Rapa for 4 hours. d and h, Immunoblots of the indicated proteins in lysates of WT and mkk1/2Δ cells cultured ± 5 µg/ml Tm or 0.2 µg/ml Rapa for 4 hours. e and f, Immunoblots of the indicated proteins in lysates of WT or mpk1Δ cells ± 50 µg/ml Congo red (CR) for 4 hours. Representative results of at least three independent experiments (biological replicates) are shown.