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. Author manuscript; available in PMC: 2017 Jan 18.
Published in final edited form as: Nat Cell Biol. 2016 Jul 18;18(8):886–896. doi: 10.1038/ncb3389

Figure 3. Yap transcriptionally upregulates GLUL in an evolutionarily conserved fashion.

Figure 3

(a) Chromatin Immunoprecipitation (ChIP) qPCR analysis of Yap enrichment at glula promoter in WT and lf:Yap adult livers. Shown is the average of 2 WT and lf:Yap adult liver chromatin preps, each derived from a pooled sample of 2 livers; see Supplementary Table 4.

(b) Luciferase GLUL reporter assay in cultured Hek293 cells expressing GFP, YAP1S127A or YAP1S94A. n=3 biologically independent replicates; see Supplementary Table 4. *p<0.05, two-sided Student's t-test, values represent the mean±SEM.

(c) Immunoblot analysis of GLUL, YAP, β-catenin and β-actin expression in HepG2 cells infected with shYAP retrovirus.

(d) Immunoblot analysis of GLUL and β-actin expression in Hep3B and HepG2 cells exposed to verteporfin (VP). Immunoblots are representative of three independent experiments. Unprocessed original scans of blots are shown in Supplementary Fig. 8.