Skip to main content
. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: Bio Protoc. 2014 Sep 20;4(18):e1243. doi: 10.21769/bioprotoc.1243

Figure 1. Example result from procedure B. Immunostaining 1st, in situ 2nd.

Figure 1

Image shows Purkinje neuron layer (cerebellar Purkinje neurons are identified by morphology) of a neurodegenerative disease mouse with anti-CD68 DAB staining (brown) and C1q mRNA NBT/BCIP staining (purple). In this image the C1q staining was underdeveloped and appears weak in order to demonstrate the distinct brown DAB staining. Images were taken using a Zeiss Axioplan II microscope.