Fig 2. UV exposed AD169 spiked milk and media.

Pasteurized breast milk or MEM culture medium were spiked with AD169 culture 1:5. 150 μL samples were irradiated for 10 seconds at 1, 2, 3 or 4 cm from the UV light source (estimated UV intensity 400, 100, 25 and 4 W/cm2 respectively). The irradiated samples were inoculated on to human foreskin fibroblasts (HFF). The presence or absence of CPE was determined for each well and calculated using the Reed and Muench Calculator. Results are presented as TCID₅₀/mL. Breast milk samples with values below the limit of detection are designated (*).