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. 2016 Aug 18;11(8):e0161204. doi: 10.1371/journal.pone.0161204

Fig 4. Bleomycin sensitivity of P. patens mutants.

Fig 4

Tissue explants of wild-type and mutant protonemal tissue were inoculated on BCDAT agar medium, supplemented with a 5-fold dilution series of bleomycin (1.6ng.ml-1 – 200ng.ml-1). Plants were grown for a period of approximately 3 weeks, and plant growth was determined by image analysis of digital photographs taken at intervals to generate the Growth Index. WT and mutant plants (8–12 explants) were inoculated on the same plates for direct comparison. Because individual experiments took place at intervals over a period of 2 years, direct comparisons cannot be made between each pair of panels, as different batches of bleomycin with varying potency were used during this time. Mutants analysed (from top) were Ppsrs2-KO; Pprtel1-KO (note the 5-fold difference in the amplitudes of the y-axes); Ppteb-KO and Pctip-KO. Representative images of plant growth are shown in S9 Fig.