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. 2016 Aug 18;11(8):e0161274. doi: 10.1371/journal.pone.0161274

Table 1. Primer/probe sets must be optimized to produce unbiased high-quality data from multiplexed ddPCR experiments.

Ri/r (i = D4S1652, r = CPT2) values and low and high 95% confidence intervals (CIs) computed from each ddPCR dataset shown in Fig 1.

Specimen Primer/ Probe set CPD Ri/r
D4S1652 CPT2 Mean 95% CI
Low High
Normal Blood Standard 0.220 0.221 1.00 0.96 1.03
Optimized 0.225 0.226 1.00 0.96 1.03
Normal FFPE tissue Standard 0.185 0.201 0.92 0.89 0.95
Optimized 0.196 0.194 1.01 0.98 1.04