Fig 3. Expression of BCAR3, IL1R1, KCNH2, PIR, and ZDHHC23 were significantly different between the low and high WBC ETV6-RUNX1-negative patient samples.

Total RNAs from ETV6-RUNX1-negative patient samples were isolated. cDNA was prepared using 1 μg total RNA and transcript levels were determined by real-time RT-PCR. Transcript levels were normalized to RPL13a and relative expression levels were calculated using the comparative Ct method (comparing all samples to the expression levels in the ETV6-RUNX1-positive REH cell line). The horizontal lines indicate the median. The P-values were calculated using the Mann-Whitney U-test and GraphPad Prism 5.0 software.