Figure 1.

Expression of ubiquitin-specific protease 4 (USP4) is downregulated in breast cancer tissues. (A) Immunohistochemistry was used to examine USP4 expression in paraffin-embedded sections from (a and b) normal breast tissues and (c and d) breast cancer tissues. (B) Ratio of USP4-positive cells in normal breast and breast cancer tissues. (C) USP4 protein levels in tumor tissues and matched normal tissue lesions, as assessed by western blot analysis. T, breast cancer tissues; N, normal breast tissues. Scale bars indicate 500 μm. ^**P<0.01, based on the Student's t-test. All results are from 3 or 4 independent experiments. Error bars indicate the means ± standard deviation.