Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 19;38(3):803–811. doi: 10.3892/ijmm.2016.2685

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Ubiquitin-specific protease 4 (USP4) upregulates PDCD4 expression in breast cancer cells. (A) Protein level of PDCD4 was measured in breast cancer cells after PDCD4 overexpression or silencing by western blot analysis. (B) mRNA level of PDCD4 was measured in breast cancer cells after PDCD4 overexpression or silencing by RT-qPCR. (C) HA-USP4 is specifically co-immunoprecipitated with Flag-PDCD4 in 293T cell. In co-immunoprecipitated experiments, 5% of extracts used were shown as loading controls (WCL). (D) Proteasome inhibition by MG132 suppresses the reduction of PDCD4 by USP4 silencing in MCF7 cells.