Table 1. Comparison of the secondary structure of the three proteins based on the far-UV CD spectra at pH 7 (BeStSel [46]) with the secondary structure observed in the crystal structure of BoNT/A (3BTA) (STRIDE PDB).

	BeStSel				STRIDE PDB
	LC	HN	LC-HN	LC + HN isolated	LC	HN	LC-HN	LC + HN isolated
alpha	28.3	51.8	39.6	40.05	30.2	50.2	40.7	40.2
beta	17.3	7	14.2	12.15	14	1.4	9.7	7.7
Others1	54.4	41.2	46.2	47.8	55.8	48.4	49.6	52.1

¹Others: turns and random coils