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Cell apoptosis was measured by Annexin V-fluorescein isothiocyanate (FITC) staining. Human neutrophils were pre-treated with or without 100 nM ghrelin for 1.5 h and then incubated with or without 100 ng/ml lipopolysaccharide (LPS) for 8 h. (A) Dot graphs show the flowcytometric analysis of the apoptosis of the LPS- and ghrelin-treated neutrophils. (B) Quantitative analysis of neutrophils undergoing apoptosis. *P<0.05 compared with the control.
