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. 2016 Jul 6;38(3):794–802. doi: 10.3892/ijmm.2016.2668

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of drugs on mitochondrial transmembrane potential (ΔΨm) in neutrophils. Cells were pre-treated with or without 100 nM ghrelin for 1.5 h and then simulated with or without 100 ng/ml lipopolysaccharide (LPS) for 8 h. (A) Dot graphs show the flowcytometric ananlysis of the mitochondrial transmembrane potential (ΔΨm) of the LPS- and ghrelin-treated neutrophils. ΔΨm was then determined with the lipophilic dye, JC-1. (B) Quantitative analysis of neutrophils undergoing ΔΨm. ^*P<0.05 compared with the control.