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. 2016 Aug 12;11:3875–3890. doi: 10.2147/IJN.S108689

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© 2016 Li et al. This work is published and licensed by Dove Medical Press Limited

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Cellular uptake mechanism investigated by (A) CLSM and (B) inverted fluorescence microscopy.

Notes: Uptake pathways of DOX-BSA-V-NPs in BGC-823 cells studied by using (A) LysoTracker Green (400× magnification) and (B) endocytic inhibitors (100× magnification). (A,1) DAPI; (A,2) RBITC-labeled BSA-V-NPs; (A,3) LysoTracker Green; (A,4) merge.

Abbreviations: BSA, bovine serum albumin; CLSM, confocal laser scanning microscopy; DAPI, 4′,6-diamidino-2-phenylindole; DOX, doxorubicin; RBITC, rhodamin B isothiocyanate; NPs, nanoparticles; V, vanillin.