Figure 3.

Effect of miR-34a on BCSCs is associated with a decrease in Notch1 and Hes1 expression. (A) Schematic of the Notch1 3′UTR indicates seed-matching sequences and miR-34a seed sequences. The black vertical bars indicate possible base pairing. (B, C) mRNA levels of Notch1, Hes1, c-myc, Sox2, Oct4 and Nanog under different conditions were analyzed by RT-PCR and qPCR (n = 4). Results are shown as the mean expression values normalized against miR-NC, LipoJet or DSMO. (D, E) Notch1(300KDa), Notch1(120KDa, ICN), Sox2, Oct4, Nanog and GAPDH in BCSCs transfected with miR-34a, anti-34a or inhibited by RO4929097 were detected by Western blot. (n = 3). #P<0.05.