Figure 4.

Over expression of miR-34a enhances the cytotoxic susceptibility of BCSCs to NK cell. (A) Co-culture supernatants of NK cells and BCSCs transfected with miR-34a or miR-NC were collected and the cytokine levels were detected by the mouse cytokine array panel. (B) Rae1, H60, CD155, CD112, Qa-1, H-2K and H-2D mRNA levels were analyzed by RT-PCR and qPCR under different conditions. (C) Protein was extracted from spheres treated with miR-NC, miR-34a and RO4929097, then validated the expression of H60, Qa-1 by Western blot. #P < 0.05.