Figure 2.

2ME2-induced death by apoptosis in AML cell lines. (A) Two AML cell lines were incubated with increasing doses of 2ME2 (0.08-11.25μM). After 24, 48, or 72 hours, cell viability was detected by an MTT assay, each value represents the mean ± SEM of the results of 3 independent experiments. The concentration of 2ME2 (X)=log(X). IC₅₀ for 2ME2 is shown in (B). (C) AML cell lines were incubated with 2.5μM of 2ME2 for 48 hours. Apoptosis was detected by annexin V-FITC/propidium iodide double staining. Percentages of cells showing apoptosis (combined right quadrants) are shown in the boxes. (D) Summary of the results of annexin V–FITC/7-AAD double staining from 3 independent experiments. **P < .001. Ns indicates no significant. (E) HL-60 and Kasumi-1 cells were treated with different concentration of 2ME2 (0.1, 0.5, 2.5, or 5μM) for 24 hours or diluent control, and intracelluar protein levels were examined by Western blot.